10.6084/m9.figshare.7939079.v1 Bei Liu Bei Liu Visualization L4.avi Optica Publishing Group 2019 Imaging Systems and Microscopy 2019-07-03 16:22:53 Media https://opticapublishing.figshare.com/articles/media/Visualization_L4_avi/7939079 Combined vaTIRF imaging of Paxillin and localized photoactivation of PA-Rac1. Mouse Embryonic Fibroblast (MEF) cell stably expressing photoactivatable Rac1 (PA-Rac1) transiently transfected with mCherry-Paxillin, an adhesion marker. mCherry-Paxillin was imaged using vaTIRF for a total of 20 min and the cell was locally irradiated (dashed circle) with pulsed blue light for 1 second every 5 seconds (500 µW) between the 5 and 15 min mark (blue-filled circle on the top of each panel indicates photoactivation).