Supplemental Movie 1.avi

3D projection of induced THG signals at discrete focal planes. A 3D stack was acquired beginning at z = 90 µm into the PAA gel and capturing XY images every 3 µm to a final depth of 210 µm from the coverglass (z = 0 µm) using 1280 nm excitation to detect THG signals, post activation with 1090 nm. A z-projection with rotation about the y axis shows focally localized THG signals in the ND-Cy3 containing PAA gels. Frame rate of 2fps.