Supplemental Movie 1.avi (3.12 MB)

Supplemental Movie 1.avi

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posted on 13.06.2019 by Dmitry Nevozhay, Michael Weiger, Peter Friedl, Konstantin Sokolov
3D projection of induced THG signals at discrete focal planes. A 3D stack was acquired beginning at z = 90 µm into the PAA gel and capturing XY images every 3 µm to a final depth of 210 µm from the coverglass (z = 0 µm) using 1280 nm excitation to detect THG signals, post activation with 1090 nm. A z-projection with rotation about the y axis shows focally localized THG signals in the ND-Cy3 containing PAA gels. Frame rate of 2fps.

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