Visualization2_2P_hipp_500µm_30sec-5sClip.avi

Visualization 2. Geometrically corrected volume reconstruction after application of affine transformation to the acquired stack of 600 frames captured by 2P SOPi system to cover a volume of 750×270×500 µm3 in a fixed 1 mm thick slice of Thy1GFP transgenic line mouse hippocampus (50 ms exposure time per frame, 30 seconds acquisition time). Compared to 1P imaging shown in Visualization 1 for the same brain slice, the 2P light-sheet provides better contrast and resolution of the scanned volume.