Visualization 2: Robust real-time 3D single-particle tracking using a dynamically moving laser spot

Real-time movie of a gQD@SiO2 particle being tracked by 3D-DyPLoT. The 3D plot is the movement of the piezoelectric stage to hold the diffusing gQD@SiO2 in the objective focal volume. The inset is the sCMOS readout (3x3 Gaussian filter, false-color). Originally published in Optics Letters on 15 June 2017 (ol-42-12-2390)