Visualization L4.avi

Combined vaTIRF imaging of Paxillin and localized photoactivation of PA-Rac1. Mouse Embryonic Fibroblast (MEF) cell stably expressing photoactivatable Rac1 (PA-Rac1) transiently transfected with mCherry-Paxillin, an adhesion marker. mCherry-Paxillin was imaged using vaTIRF for a total of 20 min and the cell was locally irradiated (dashed circle) with pulsed blue light for 1 second every 5 seconds (500 µW) between the 5 and 15 min mark (blue-filled circle on the top of each panel indicates photoactivation).