SupplementaryVideo5.mp4 (2.17 MB)


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posted on 13.09.2021, 15:09 by Matteo Beranrdello, maria marsal, Emilio Gualda Manzano, Pablo Loza-Alvarez
Video formed by three movies in the same area of a dclk2-GFP embryo. The z-stack (from 00:00 to 00:09) shows another internal MT bundle (arrowhead) connected to an eYSN. A 3D rotating projection of the same area (from 00:10 to 00:12) shows the extension of the same MT bundle toward the interior of the yolk, and again its connection to the eYSN (arrowhead). The same area (from 00:13 to 00:18), visualized through a maximum intensity projection over 20 µm in depth, shows the evolution of the connection between the internal MT bundles and the eYSN versus time. The connections (arrowheads) are lost upon eYSN mitosis and reform with the resulting nuclei. Orange HOT colormap is used to ease visualization of the internal MT bundle. Time stamp in minutes : seconds. Scale bar 50 µm.


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