mediaposted on 12.07.2018, 16:45 by Peter Quicke, Stephanie Reynolds, Mark Neil, Thomas Knopfel, Simon Schultz, Amanda Foust
A source localized (top) and non-source localized (bottom) image of a GFP-expressing neuron's apical dendrite acquired with the multifocal two-photon system. Fluorescence was excited by a linear array of beamlets rastered with the rectangle's long axis perpendicular to the beamlet line's axis. The camera acquired one frame per vertical sweep to generate a set of 8 streak images. Complete frames were recovered by taking a maximum intensity projection through each set of 8 streak images.