PSO-AO correction on a single 200nm yellow-green fluorescent microsphere. The experiment was performed with particles placed in the position range from -5 to 5 radians. The velocity range was -0.5 to 0.5. The population size was 50, and the optimization ran for 50 generations. Values of r1 and r2 were both 2.0. Sigma for the metric was 0.1 μm-1. The exposure time was 50 ms. A 470 nm LED was used for illumination. Scale bars are 1 µm. The video shows all 2500 images acquired in sequence during the optimization. This video corresponds to the data in Fig. 1.