A multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality

Published on 2020-06-29T16:05:00Z (GMT) by
Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation can be combined with other nonlinear techniques, such as coherent Raman spectroscopy which typically allow chemical imaging of a single resonant vibrational mode of a sample. Here, we utilize a single ultrafast laser source to obtain broadband coherent Raman spectra on a microscope, together with other nonlinear microscopy approaches on the same instrument. We demonstrate that the coherent Raman modality allows broadband measurement (>1000 cm-1), with high spectral resolution (<5 cm-1), with a rapid spectral acquisition rate (3-12 kHz). This enables Raman hyperspectral imaging of > kilo-pixel images at >11 frames per second.

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Ideguchi, Takuro; Sinjab, Faris; Hashimoto, Kazuki; Ramaiah-Badarla, Venkata; Omachi, Junko (2020): A multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.4996463.v1