Correlative cross-cumulant and radiality microscopy for multicolor super-resolution subcellular imaging
Published on 2020-05-20T22:10:18Z (GMT) by
Fluorescence fluctuation-based super-resolution techniques can achieve fast super-resolution imaging on a cost-effective wide-field platform at a low light level with reduced phototoxicity. However, the current methods exhibit certain imaging deficiencies that misinterpret nanoscale features reconstructed from fluctuating image sequences, thus degrading the reconstructed image fidelity and quality. Here we propose correlative cross-cumulant and radiality microscopy (COXCURM) that employs the cross-cumulant analysis in tandem with radiality processing. We demonstrated that COXCURM can significantly improve the spatial resolution at a low light level whilst eliminate the misinterpretations of nanoscale features of the existing fluctuation-based super-resolution methods. In the experiment, we further verified the superior performance of COXCURM over the current methods through performing multicolor super-resolution imaging of subcellular microtubule networks and clathrin-coated pits (CCPs) as well as the high-precision reconstruction of densely packed RNA transcripts.
Cite this collection
Zeng, Zhiping; Ma, Jing; Canhua, Xu (2020): Correlative cross-cumulant and radiality microscopy for multicolor super-resolution subcellular imaging. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.4864326.v1