Development and characterization of disposable sub-millimeter fiberoptic Raman needle probe for real-time in vivo deep tissue and biofluids Raman measurements

Posted on 12.10.2021 - 13:08
We report on the development and characterization of disposable sub-millimeter fiberoptic Raman needle probe for real-time in vivo tissue and biofluids Raman measurements. The sub-millimeter Raman probe is designed and fabricated using an aluminum-coated multimode fiber tapered with a semi-spherical lens, resulting in the coaxial laser excitation/Raman collection configuration for maximizing tissue and biofluid Raman measurements. We demonstrate that with the use of the Raman needle probe associated with fiber silica Raman/fluorescence background subtraction algorithms developed, high quality tissue Raman spectra covering both the fingerprint (FP) (800-1800 cm-1) and high-wavenumber (HW) (2800-3300 cm-1) regions can be acquired within sub-seconds from different tissue types (e.g., skin, muscle, fat, cartilage, liver and brain) and biofluids (e.g., blood, urine). By advancing the Raman needle probe into the murine brain tissue model, high quality depth-resolved deep tissue Raman spectra can also be acquired rapidly. This work shows that the sub-millimeter fiberoptic Raman needle probe is capable of achieving real-time collection of deep tissue and biofluids FP/HW Raman spectra with high signal to noise ratios, suggesting its potential applications in dual functioning of Raman optical biopsy and fine needle aspiration biopsy for in vivo deep tissue and biofluids diagnosis and characterization in different organs.

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Huang, Zhiwei; Shu, Chi; Zheng, Wei; Wang, Zhuo; Yu, Changyuan (2021): Development and characterization of disposable sub-millimeter fiberoptic Raman needle probe for real-time in vivo deep tissue and biofluids Raman measurements. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5629669.v2
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