Femtosecond Diode Based Time Lens Laser for Multiphoton Microscopy

Posted on 14.09.2021 - 17:38
We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 μm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.


Simmons, Yonge; Underwood, Kenneth; Supekar, Omkar; Heffernan, Brendan; Welton, Tarah; Gibson, Emily; et al. (2021): Femtosecond Diode Based Time Lens Laser for Multiphoton Microscopy. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.5412477
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Biomedical Optics Express


Yonge Simmons
Kenneth Underwood
Omkar Supekar
Brendan Heffernan
Tarah Welton
Emily Gibson
Juliet Gopinath


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