Heterodyne Brillouin microscopy for biomechanical imaging

Posted on 14.09.2021 - 16:17
Microscopic variations in material stiffness play a very important role in cellular scale biomechanics, but are difficult to measure in a natural 3D environment. Brillouin microscopy is a promising technology for such applications which provides non-contact label-free measurement of mechanical properties at microscopic resolution. Here we develop heterodyne detection to measure Brillouin scattering signals in a confocal microscope setup, providing sensitive detection with excellent frequency resolution and robust operation in the presence of stray light. The functionality of the microscope is characterized and validated, and the imaging capability demonstrated by imaging structure within both a fibrin fiber network and live cells.

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Taylor, Michael; Kijas, Amanda; Wang, Zhao; Lauko, Jan; Rowan, Alan (2021): Heterodyne Brillouin microscopy for biomechanical imaging. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.5599152.v2
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Biomedical Optics Express

AUTHORS (5)

Michael Taylor
Amanda Kijas
Zhao Wang
Jan Lauko
Alan Rowan

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