In vivo longitudinal visualization of the brain neuroinflammatory response at the cellular level in LysM-GFP mice induced by 3-nitropropionic acid
Posted on 2020-07-31 - 15:44
Blood-brain barrier (BBB) dysfunction is related to the development of
neuroinflammation in the central nervous system (CNS). Neuroinflammation has been
implicated as one of the key factors in the pathogenesis of neurodegenerative diseases such as
Alzheimer’s disease, Huntington’s disease, and Parkinson’s disease. Despite its importance,
the impacts and underlying cellular mechanisms of chronic BBB impairment in
neurodegenerative diseases are poorly understood. In this work, we performed a longitudinal
intravital brain imaging of mouse model with neuroinflammation induced by 3-nitropropionic
acid (3-NP). For this, we obtained a transgenic LysM-GFP mouse expressing the green
fluorescence protein (GFP) in a subset of leukocytes. By using intravenously injected
fluorescence blood tracers, we longitudinally observed in vivo dynamic cellular behaviors and
the BBB integrity through a 30-day neuroinflammatory state. Vascular leakages in the
cerebral cortex reflecting BBB impairment were observed at two weeks, which persisted to
the third week, followed by a severe inflammatory response with massive leukocytes
infiltration at day 30. These descriptions can help in the development of novel approaches to
treat neurodegenerative conditions.
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Lee, Jingu; Kong, Eunji; Hong, Sujung; Moon, Jieun; Kim, Pilhan (2020). In vivo longitudinal visualization of the brain neuroinflammatory response at the cellular level in LysM-GFP mice induced by 3-nitropropionic acid. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4907220.v1
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AUTHORS (5)
JL
Jingu Lee
EK
Eunji Kong
SH
Sujung Hong
JM
Jieun Moon
PK
Pilhan Kim