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Lattice light sheet microscopy using tiling lattice light sheets

Posted on 2019-01-16 - 20:22
We present a novel method to implement tiling lattice light sheets (LLS) in Lattice light sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS microscope hardware. A LLS is tiled by applying binary phase maps acquired from off-center cross-sections of the corresponding optical lattice to the binary SLM used in LLS microscopes, by which a thin LLS can be tiled to image large specimens while maintaining the 3D imaging ability in the entire field of view. We investigate the method via numerical simulations and experiments, and demonstrate the method by imaging fluorescent particles embedded in agarose gel and expanded cells.

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AUTHORS (4)

Liang Gao
Wei-Chun Tang
Yun-Chi Tsai
Bi-Chang Chen

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