Pitch-rotational manipulation of single cells and
particles using single-beam thermo-optical
tweezers
Posted on 2020-06-08 - 14:15
3D pitch rotation of microparticles and cells assumes importance in a wide variety
of applications in biology, physics, chemistry and medicine. Applications such as cell imaging
and injection benefit from pitch-rotational manipulation. Generation of such motion in single
beam optical tweezers has remained elusive due to complicacies of generating high enough
ellipticity perpendicular to the direction of propagation. Further, trapping an extended object
at two locations can only generate partial pitch motion by moving one of the foci in the axial
direction. Here, we use hexagonal-shaped upconverting particles and single cells trapped close
to a gold-coated glass cover slip in a sample chamber to generate complete 360 degree and
continuous pitch motion even with a single optical tweezers beam. The tweezers beam passing
through the gold surface is partially absorbed and generates a hot-spot to produce circulatory
convective flows in the vicinity which rotates the objects. The rotation rate can be controlled by
the intensity of the laser light and the thickness of the gold layer. Thus such a simple configuration
can turn the particle in the pitch sense. The circulatory flows in this technique have a diameter of
about 5 µm which is smaller than those reported using acousto-fluidic techniques.
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kumar, sumeet; M, Gunaseelan; Vaipully, Rahul; Kumar, Amrendra; Ajith, Mithun; Vaidya, Gaurav; et al. (2020). Pitch-rotational manipulation of single cells and
particles using single-beam thermo-optical
tweezers. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4894497.v1
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AUTHORS (8)
sk
sumeet kumar
GM
Gunaseelan M
RV
Rahul Vaipully
AK
Amrendra Kumar
MA
Mithun Ajith
GV
Gaurav Vaidya
Sd
Soumya dutta
BR
Basudev Roy