Quantitative third-harmonic generation
imaging of mouse visual cortex areas reveals
correlations between functional maps and
structural substrates
Posted on 2020-09-16 - 15:46
The structure of brain regions is assumed to correlate with their function, but there
are very few instances in which the relationship has been demonstrated in the live brain. This
is due to the difficulty of simultaneously measuring functional and structural properties of
brain areas, particularly at cellular resolution. Here, we performed label-free, third-harmonic
generation (THG) microscopy to obtain a key structural signature of cortical areas, their
effective attenuation lengths (EAL), in the vertical columns of functionally defined primary
visual cortex and five adjacent visual areas in awake mice. EALs measured by THG
microscopy in the cortex and white matter showed remarkable correspondence with the
functional retinotopic sign map of each area. Structural features such as cytoarchitecture,
myeloarchitecture and blood vessel architecture were correlated with areal EAL values,
suggesting that EAL is a function of these structural features as an optical property of these
areas. These results demonstrate for the first time a strong relationship between structural
substrates of visual cortical areas and their functional representation maps in vivo. This study
may also help in understanding the coupling between structure and function in other animal
models as well as in humans.
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Yildirim, Murat; So, Peter; Hu, Ming; Sur, Mriganka; Sugihara, Hiroki; Le, Nhat (2020). Quantitative third-harmonic generation
imaging of mouse visual cortex areas reveals
correlations between functional maps and
structural substrates. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4963256.v1
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AUTHORS (6)
MY
Murat Yildirim
PS
Peter So
MH
Ming Hu
MS
Mriganka Sur
HS
Hiroki Sugihara
NL
Nhat Le