Scattering oblique plane microscopy for in-vivo blood cell imaging

Posted on 05.04.2021 - 16:26
Oblique plane microscopy (OPM) enables high speed, volumetric fluorescence imaging through a single-objective geometry. While these advantages have positioned OPM as a valuable tool to probe biological questions in animal models, its potential for in-vivo human imaging is largely unexplored due to its typical use with exogenous fluorescent dyes. Here we introduce a scattering-contrast oblique plane microscope (sOPM) and demonstrate label-free imaging of blood cells flowing through human capillaries \textit{in vivo}. The sOPM illuminates a capillary bed in the ventral tongue with an oblique light sheet, and images side- and back-scattered signal from blood cells. By synchronizing sOPM with a conventional capillaroscope, we acquire paired widefield and axial images of blood cells flowing through a capillary loop. The widefield capillaroscope image provides absorption contrast and confirms the presence of red blood cells (RBCs), while the sOPM image may aid in determining whether optical absorption gaps (OAGs) between RBCs have cellular or acellular composition. Further, we demonstrate consequential differences between fluorescence and scattering versions of OPM by imaging the same polystyrene beads sequentially with each technique. Lastly, we substantiate in-vivo observations by imaging isolated red blood cells, white blood cells, and platelets in-vitro using 3D agar phantoms. These results demonstrate a promising new avenue towards in-vivo blood analysis.

CITE THIS COLLECTION

Select your citation style and then place your mouse over the citation text to select it.
McKay, Gregory; Niemeier, Ryan; Castro-González, Carlos; Durr, Nicholas (2021): Scattering oblique plane microscopy for in-vivo blood cell imaging. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.5344502.v1
or

SHARE

email
Biomedical Optics Express

AUTHORS (4)

Gregory McKay
Ryan Niemeier
Carlos Castro-González
Nicholas Durr

KEYWORDS

need help?