Spatial frequency-based image reconstruction to improve image contrast in multi-offset adaptive optics ophthalmoscopy
Posted on 23.02.2021 - 15:09
Off-axis detection methods in adaptive optics (AO) ophthalmoscopy can enhance image contrast of translucent retinal structures such as cone inner segments and retinal ganglion cells layer neurons. Here, we propose a 2D optical model showing that the phase contrast produced by these methods depends on the offset orientation. While one axis provides an asymmetric light distribution, hence a high phase contrast, the perpendicular axis provides a symmetric one, thus a substantially lower contrast. We support this model with in-vivo human data acquired with a multi-offset AO scanning light ophthalmoscope. Then, using this finding, we provide a post-processing method, named Spatial frequency-based iMAge ReconsTruction (SMART), to optimally combine images from different off-axis detector orientations, significantly increasing the structural cellular contrast of in-vivo human retinal neurons such as cone inner segment, putative rods and retinal ganglion cells.
CITE THIS COLLECTION
Select your citation style and then place your mouse over the citation text to select it.
Mece, Pedro; Gofas-Salas, Elena; Rui, Yuhua; Zhang, Min; Sahel, José-Alain; Rossi, Ethan (2021): Spatial frequency-based image reconstruction to improve image contrast in multi-offset adaptive optics ophthalmoscopy. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.5242049.v1
Read the peer-reviewed publication