Spatial frequency-based image reconstruction to improve image contrast in multi-offset adaptive optics ophthalmoscopy

Posted on 23.02.2021 - 15:09
Off-axis detection methods in adaptive optics (AO) ophthalmoscopy can enhance image contrast of translucent retinal structures such as cone inner segments and retinal ganglion cells layer neurons. Here, we propose a 2D optical model showing that the phase contrast produced by these methods depends on the offset orientation. While one axis provides an asymmetric light distribution, hence a high phase contrast, the perpendicular axis provides a symmetric one, thus a substantially lower contrast. We support this model with in-vivo human data acquired with a multi-offset AO scanning light ophthalmoscope. Then, using this finding, we provide a post-processing method, named Spatial frequency-based iMAge ReconsTruction (SMART), to optimally combine images from different off-axis detector orientations, significantly increasing the structural cellular contrast of in-vivo human retinal neurons such as cone inner segment, putative rods and retinal ganglion cells.

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Mece, Pedro; Gofas-Salas, Elena; Rui, Yuhua; Zhang, Min; Sahel, José-Alain; Rossi, Ethan (2021): Spatial frequency-based image reconstruction to improve image contrast in multi-offset adaptive optics ophthalmoscopy. The Optical Society. Collection. https://doi.org/10.6084/m9.figshare.c.5242049.v1
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AUTHORS (6)

Pedro Mece
Elena Gofas-Salas
Yuhua Rui
Min Zhang
José-Alain Sahel
Ethan Rossi
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