Splicing Exponential point spread function design for localization of nanoparticles
Posted on 13.10.2021 - 16:25
We propose a point spread function for three-dimensional localization of nanoparticles. The axial detection range of the point spread function can be simply changed by adjusting the design parameters. In addition, the maximum size of the point spread function can also be changed by adjusting the design parameters, which is an advantage other point spread functions do not have. We used our point spread functions and the existing point spread functions for dense multi-particle imaging, which proved the advantage that the point spread function with a smaller maximum size we designed can effectively reduce the overlap between the point spread functions. The three-dimensional process of fluorescent microspheres penetrating HT-22 cell membrane was successfully recorded by SE-PSF, which verified the effectiveness of this method.
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Li, Hangfeng; Wang, Famin; Miu, Xin; HUANG, Wei; Cheng, Yu; Xiao, Yun; et al. (2021): Splicing Exponential point spread function design for localization of nanoparticles. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5561346.v3
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