Three-dimensional dynamic optical coherence tomography for tumor spheroid evaluation

Posted on 12.10.2021 - 14:45
We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.

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El-Sadek, Ibrahim Abd; Miyazawa, Arata; Shen, Larina Tzu-Wei; Makita, Shuichi; MUKHERJEE, PRADIPTA; Lichtenegger, Antonia; et al. (2021): Three-dimensional dynamic optical coherence tomography for tumor spheroid evaluation. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5641864.v2
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AUTHORS (8)

Ibrahim Abd El-Sadek
Arata Miyazawa
Larina Tzu-Wei Shen
Shuichi Makita
PRADIPTA MUKHERJEE
Antonia Lichtenegger
Satoshi Matsusaka
Yoshiaki Yasuno

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