Tunable SIM: observation at varying spatiotemporal resolutions across the FOV
Version 2 2020-08-07, 17:06
Version 1 2020-08-07, 17:05
Posted on 2020-08-07 - 17:06
To date, imaging systems have generally been designed to provide an even spatiotemporal resolution across the field of view (FOV). However, this becomes a fundamental limitation when we aim to simultaneously observe varying dynamics at different parts of the FOV. In conventional imaging systems, to capture fast dynamics occurring at only a small portion of the FOV, the entire imaging system’s sampling rate must be increased. This is a major problem if different parts of the FOV must rather be imaged at high spatial resolutions beyond the diffraction limit and require a sacrifice in temporal resolution. To answer this unmet challenge, we propose tunable SIM, which enables adaptive modulation of spatiotemporally varying structured illumination across different parts of the FOV. Using tunable SIM, we exploit the varying and designable spatiotemporal resolution to demonstrate simultaneous measurements of sub-diffraction-limited changes in the actin fine structure of U87MG-EGFP-CD9 cells and the fast viscous flow inducing these structural changes.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
WOO, TAESEONG; Jeong, Soohyun; ahn, cheolwoo; Hwang, Byungjae; kim, hyunggee; Kang, JooHun; et al. (2020). Tunable SIM: observation at varying spatiotemporal resolutions across the FOV. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4893039.v2
or
Select your citation style and then place your mouse over the citation text to select it.
Resource Link
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (7)
TW
TAESEONG WOO
SJ
Soohyun Jeong
ca
cheolwoo ahn
BH
Byungjae Hwang
hk
hyunggee kim
JK
JooHun Kang
JP
Jung-Hoon Park