precise measurement of pure dielectrophoresis force acting on a RBC using optical tweezers

The force experienced by a neutral dielectric object in the presence of a spatially non-uniform electric field is referred to as dielectrophoresis (DEP). The precise quantification of DEP force in the single-cell level could of great importance in designing high-efficiency micro- fluidic systems for separation of biological cells. In this report we show how optical tweezers can be properly utilized for precise quantification of DEP force experienced by a human RBC. By tuning the temporal frequency of the applied electric field and also performing control experiments and comparing our experimental results with that of theoretically calculated, we show that the measured force is a pure DEP force. Our results show that in the frequency range of 0.1-3 M H z the DEP force acting on RBC is frequency independent.